FLUORESCENCE PROBE SENSITIVE TO DETECT G-RICH TARGET STRANDS THROUGH QUENCHING

© 2009 Arvind Misra^#, Pratibha Dwivedi, Mohammad Shahid

^#Tel.: +91-542-2307321 ext. 104; fax: +91-542-2368127; e-mail: [email protected]

Nucleic Acids Research Laboratory, Department of Chemistry, Faculty of Science, Banaras Hindu University, 221 005, Varanasi, India

Received: May 05, 2008; in final form: August 31, 2008

Modified fluorescent probe U^FAA AAT CTC CGC CGC has been synthesized using nucleoside analogues viz. 3’-O-(N,N’-Diisopropylamino-2-cyanoethoxyphosphinyl)-5’-O-(4,4’-dimethoxytrityl)-2’-O-(dansyl-1-sulfonamidohexylaminocarbonyl) uridine for hybridization studies with perfectly matched (U/A) complementary and with DNA strand having similar ‘G’ rich telomeric units at the 3’-end. The thermal stability data and decrease in fluorescence intensity due to the presence of ‘dG’ units have clearly demonstrated the potential application of current approach in DNA diagnostics under homogeneous hybridization assays.

Key words: telomere, quenching, Deoxyguanosine.