DEVELOPMENT, OPTIMIZATION, AND BIOVALIDATION OF ^99mTc–INSULIN COMPLEX

© 2016 Muhammad Usman Akbar*^, **^{, #}, Tanveer Hussain Bokhari*, Samina Roohi***, Khalid Mahmood Zia**, Mohammad Zuber**, Nadia Parveen****, and Narmeen Ali*

^#Phone: 0092-333-6526411; e-mail: [email protected]

*Department of Chemistry, Government College University, Faisalabad, 38000 Pakistan;
**Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, 38000 Pakistan;
***Isotope Production Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, Pakistan;
****Pakistan School Muscat Sultanate of Oman

Received December 7, 2015; in final form, April 19, 2016

DOI: 10.7868/S0132342316050134

Human biosynthetic insulin is a polypeptide hormone that plays an important and essential role in control of the level of carbohydrate, protein, and fat metabolism in the blood. Human pancreatic insulin was labeled with ^99mTc to form a new radiopharmaceutical with a labeling yield of 99 ± 1% under optimum conditions: 0.1 mL insulin, pH 7, 25 μg stannous chloride, 1 mL (19 mCi) of pertechnetate, room temperature, and 10 min reaction time. The ^99mTc–insulin complex was examined using paper chromatography, ITLC, electrophoresis, and HPLC. In addition, in vitro and in vivo study of ^99mTc–insulin complex was performed at different

time intervals.

Keywords: insulin, technetium-99m, labeling, optimization, HPLC, electrophoresis, biodistribution.

Биоорг. химия 2016, 42 (5): 546-551