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SYNTHESIS OF N-GLYCOPROTE1NS WITH A NATURAL TYPE OF CARBOHYDRATE-PEPTIDE BOND
LIKHOSHEESTOV L. M., PISKAREV V. E., DEREVITSKAYA V. A., KOCHETKOV N. K.
N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: Reaction of β-oligoglycosylamines obtained from carbohydrate chains of N-gly-coproteins (ovalbumin, ovomucoid, riboflavin-binding glycoprotein from hen egg white, and asialofetuin) with bovine serum albumin, lysozyme, and poly(Z,-Asp) in presence of water-soluble carbodiimide gave rise to a series of glycoconjugates, modelling natural K-glycoproteins. Carbohydrate-peptide bond was shown to be of N-glycosylamide type with participation of Asp and Glu residues. The method allows to obtain synthetic N-glycoproteins from oligomannoside, complex and hybride oligosaccharide chains, and may find application both in biochemistry and biotechnology for improvement of physico-chemical properties of unglycosylated proteins.
Russian Journal of Bioorganic Chemistry 1990, 16 (1):118-120