SYNTHESIS AND IMMUNOLOGIC PROPERTIES OF A PEPTIDE FRAGMENT OF THE HEPATITIS B ENVELOPE preS-PROTEIN

EVSTIGNEEVA. P., ZHELTUKHINA G. A., PROKURONOVA ?. I., SMIRNOV V. D.*, SEMILETOV Yu. A.*, KALININA T. I.*, KHUDYAKOV Yu. E.*, KHROMOV I. S.*, FAVOROV M. O.*, YASHINA T. L.*

M. V. Lomonosov Institute of Chemical Technology, Moscow; *D.I. Ivanovskii Institute of Virology, Academy of Medical Sciences of the USSR, Moscow

Abstract: We have synthesized the 24-41 fragment of the preS region of the hepatitis B (subtype ayw) envelope. The peptide was prepared by the solid phase synthesis on perfluoropoly-ethylene polymer grafted with polystyrene. The peptide chain was elongated from C-terminus using pentafluorophenyl- and p-nitrophenyl esters of Boc-amino acids. The peptide was cleaved off the solid phase by HBr in CF₃COOH, purified by gel filtration, and after conjugation with serum albumin (BSA) inoculated into mice. The resultant antibodies were shown to react with the peptide. The blood sera from patients with acute hepatitis B reacted with the peptide-BSA conjugates in the immunoenzymic solid phase assay.

Russian Journal of Bioorganic Chemistry 1990, 16 (1):34-40

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