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Gel permeation HPLC partial purification of glycosyl transferases catalysing the Salmonella anatum and S. kentucky O-antigens biosynthesis
T. N. Druzhinina, O. V. Sizova, V. I. Torgov, V. N. Shibaev
N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: The solubilized glycosyltransferases which catalyse the biosynthesis of Salmonella anatum arid S. kentucky O-specific polysaccharides were partially purified by HPLC on Superose 12. Two mannosyl transferases from S. kentucky were separated by gel chromatography; these transferases were found useful for chemical-enzymic synthesis of polyprenylpyrophosphate derivatives of trisaccharides Tal-Man-Gal and Man-Tal-Gal.
Russian Journal of Bioorganic Chemistry 1990, 16 (10):1325-1331