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Investigation and immunoanalytical application of peroxidasc from Arthromyces ramosus
B. B. Kim, V. V. Pisarev, S. B. Vlasenko, A. M. Egorov
M. V. Lomonosov State University, Moscow
Abstract: Some biochemical and catalytic properties of peroxidase from Arthromyces ramosus (EC 1.11.7.1) in chemiluminescent reaction of luminol oxidation by hydrogen peroxide were investigated. The second order rate constants were determined by the stopped-flow technique. Optimal conditions to quantity the enzyme were found, the detection limit being 5·10-13 M. The peroxidase was used as a marker in the human IgG immunoassay.
Russian Journal of Bioorganic Chemistry 1990, 16 (10):1332-1338