Plasmid expression vectors utilizing fragments of the E. coli alkaline phosphatase gene

S. A. Zozulya, T. A. Obukhova, E. P. Shirokova, P. R. Badalov

Branch of M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow Region

Abstract: A family of plasmid expression vectors, containing fragments of the E. coli alkaline phosphatase gene (phoA), has been constructed for the λP_R promoter-directed thermoindu-cible superproduction of fusions of heterologous polypeptides to the N- or C-terminus of E. coli alkaline phosphatase and its leader peptide. Effective expression and export to pe-riplasm of resulting fusion proteins, which may retain enzymatic activity of the phospha.-tase has been shown.

Russian Journal of Bioorganic Chemistry 1990, 16 (10):1339-1347

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