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Application of liquid secondary ion mass spectrometry for the quantitative determination of fusicoccin terpenoides produced by Fusicoccum amygdaly del
V. L. Sadovskaya, L. Yu. Rakitin, I. I. Grishina, L. M. Krasnopolskaya, G. S. Muromtsev, V. P. Shevchenko
All-Union Research Institute of Agricultural Biotechnology, Academy of Agricultural Sciences of the USSR, Moscow; Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow
Abstract: Application of the liquid secondary ion mass spectrometry (LSIMS) for quantitative evaluation of the fusicoccin terpenoids in the cultural liquid of Fusicoccum amygdali Del. has been studied. Function of the [M+Na]+ ion intensity ratio IC J H Ia versus the fusicoccin C, J or H amount being linear within 1–5 nmoles, fusicoccin C, J and H contents can be determined in mixtures containing known amount of fusicoccin A. For quantitative determination of fusicoccin A in the cultural liquids the isotopic dilution, method is suggested with [2H4]dihydrofusicoccin A as the internal standard.
It is shown that a rise in the relative polarity of compounds being analysed increases the LSIMS signal response.
Russian Journal of Bioorganic Chemistry 1990, 16 (10):1407-1412