3-CYANO-4-METHYLUMBELLIFERYL-β- D -GALACTOPYRANOSIDE, A NEW CHROMOGENIC AND FLUORIGENIC SUBSTRATE FOR THE β-GALACTOSIDASE FROM E.COLI

A. N. MARKARYAN, Ya. V. VOZNYI *

A. N. Bach Institute of Biochemistry, Academy of Sciences of the USSR, Moscow; *Institute of Biochemistry, Academy of Sciences of the Armenian SSR, Yerevan

Abstract: A novel substrate, 3-cyano-4-trifluoromethylumbelliferryl-β-D-galactopyranoside, for the continuous (pK _a = 6,0 for the measured product), photometric (e= 42000 M-¹ cm^-3 at 440 nm, pH 7,5) and fluorimetric (λ_ex= 440 nm, λ_em=500 nm) assay of β-galactosidase was synthesized. The substrates affinity to nitrocellulose membranes made possible its use for sensitive (up to 0,1 mole of the enzyme in 5—10 min) visual identificatioa of the β-galactosidase activity on nitrocellulose sheets.

Russian Journal of Bioorganic Chemistry 1990, 16 (4):569-570

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