Structure of carbohydrate chains of riboflavin-binding glycoprotein from hen egg white. III. ¹NMR 500 MHz spectroscopy of neutral fucosylated oligosaccharides

V. E. Piskarev, L. M. Likhosherstov, N. F. Sepetov, E. L. Galenko, V. A. Derevitskaya

N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow;

All-Union Cardiological Centre, Institute of Experimental Cardiology, Academy of Medical Sciences of the USSR, Moscow

Abstract: Using LiBH₄/Bu^tOH treatment, oligosaccharides were cleaved off the hen egg white riboflavin-binding glycoprotein. HPLC led to the isolation of four fucose-contai-ning oligosaccharide alditols, whoso structure was elucidated by means of ¹H NMR 500 MHz spectroscopy. The main fucosylated oligosaccharide, also present in hen ovo-mucoid, was found to be a biantennary carbohydrate chain of N-acetyllactosamine type.

Russian Journal of Bioorganic Chemistry 1990, 16 (7):951-955

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