Anti trans-DDP-DNA antibodies for detection of specific DNA sequences

V. I. Kiseleva, M. F. Turchinsky, A. M. Poverenny

Research Institute of Medical Riadiology, Academy of Medical Sciences of the USSR, Obninsk; M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system have been used for the hybrides visualisation. This method allows one to detect 2 pg/mm² DNA.

Russian Journal of Bioorganic Chemistry 1990, 16 (7):991-992

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