Approach to isolation of individual tRNAs from human placenta based on affinity chromatography. Isolation of highly purified Phe-tRNA^Phe

V. F. Zarytova, G. G. Karpova, D. A. Mundus, I. G. Shishkina

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences of the USSR

Abstract: A new approach to isolation of individual tRNAs from eukaryotes based on affinity chromatography is suggested. At first, using a sorbent with oligonucleotide pTGGT attached, the total tRNA with native CCA-ends was obtained. Then by means of a sorbent with oligonucleotide pTTCAG immobilized, which is complementary to a part of the tRNA^phe anticodon loop, tRNA^phe with the acceptor activity>1000 pmole/unit was isolated.

Russian Journal of Bioorganic Chemistry 1990, 16 (8):1069-1072

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