Interaction of α-[¹²⁵I]latrocrustatoxin with plasmatic membranes from the nerve cells of the crayfish Astacus astacus

V. G. Krasnoperov, O. G. Shamotienko, E. V. Grishin

Branch of the M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow Region; M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: α-Latrocrustatoxin, the crustacean-specific neurotoxin from the venom of the black widow spider Latrodectus mactans tredecimguttatus was radioactively labelled with Bol-ton – Hunter reagent to the specific activity of 160 Ci/mmol with retention of the biological activity. A highly specific binding of radioactive toxin on plasmatic membranes from the crayfish Astacus astacus nerve cells with 5_max=0,04 pmol binding toxin/mg membrane protein and K_d=0,7-10^-10 M was demonstrated.

Russian Journal of Bioorganic Chemistry 1991, 17 (5):716-718

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