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Oligodeoxynucleotides containing a HaeIII restriction site with 6-O-alkylated guanine residue(s)
M. O. Taktakishvili, A. Tabjun
Department of Chemistry, Tbilisi State University, Tbilisi
Abstract: A series of self-complementary decadeoxynucleotides containing a native or modified HaeIII site GGCC (with one or both guanine residues 6-O-cetylated) have been synthesized by the phosphotriester approach. The nonmodified decanucleotide is normally digested with snake venom phosphodiesterase as well as with HaeIII and Bspl restriction endonucleases, whereas the bulky 6-O-alkyl substitutent strongly inhibits, the VPDE hydrolysis and completely prevents digestion with the endonucleases.
Russian Journal of Bioorganic Chemistry 1991, 17 (6):806-808