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Chemical-enzymatic synthesis and cloning in Escherichia coli of genes coding for human granulocyte colony stimulating
S. K. Kashyap, N. S. Bystrov, E. F. Boldyreva, I. A. Polyakova, I. V. Severtsova, V G. Korobko
M. M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
Abstract: Two artificial genes, encoding two forms of human granulocyte colony stimulating factor as products of a normal and an alternative splicing, have been by a chemical-enzymatic way synthesized and cloned in Escherichia coli. The genes are supplied with recognition sites of restriction endonucleases to facilitate the further cassette mutagenesis.
Russian Journal of Bioorganic Chemistry 1992, 18 (1):71-77